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Paddy Ross

Osteoclast culture - techniques and applications

Topics to be covered

Methods for isolation of osteoclast precursors and use thereof to generate osteoclasts in vitro.

Methods for analysis of cytokine-stimulated osteoclast signaling.

Approaches to meaningful assay in vitro of osteoclast differentiation, morphology and function.

Use of retro- lenti- and adenoviral approaches to introduce proteins into osteoclasts and/or their precursors.
 

Educational Goals

At the end of the discussion attendees will:

  • Understand the cellular and molecular basis of osteoclast differentiation.
  • Have detailed protocols for generating osteoclasts, performing signal transduction studies and analyzing osteoclast function.
     

Target audience

Basic scientists interested in performing experiments in osteoclast biology.
 

Teaching methods

Discussion of studies performed in the laboratories of leading osteoclast researchers.

   
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