Post-doctoral position, 1-year renewable
Inserm Laboratory UMR1229 RMeS “Regenerative medicine and skeleton”
Team: REGOS “Regenerative medicine of bone tissue”
Recruiting body: University of Nantes
Funding: Agence Nationale de Recherche (France)

The position is to be filled from the beginning of 2022

The host team’s long-term goal is to demonstrate the role of extracellular vesicles (EVs) of osteocytic origin in the maintenance and physiological repair of bone tissue. Recent observations indeed suggest that osteocytes produce EVs which could constitute a sophisticated mechanism of intercellular communication in bone. The increase in bone micro-fractures during aging could both participate in compromising the mechanical properties of bone, but also in altering this communication path between osteocytes and other bone cells. Our hypotheses are that 1 / these EVs released by osteocytes transport powerful signals to other bone cells, and control osteogenesis, 2 / these EVs are altered during aging either in quantity or in quality, and 3 / these EVs and their cargos constitute new tools for bone regeneration, based on the mobilization and activation of the healing potential of the host tissue.

The candidate’s mission will be to identify and analyze the cargos that are associated with EVs (transcriptomic and proteomic analysis) isolated from young and old mice in order to highlight the signals altered during aging. Finally, by using models of bone loss mastered in the laboratory, the candidate will participate in the demonstration of the regenerative capacities of the most promising cargos.

Candidate profile: The candidate should have solid skills in transcriptomic and / or proteomic analysis and cell biology (cells in culture, transfection). For this position, experience in animal experimentation is necessary, the animal experimentation certificate “Design or realization of procedures” would be a plus.
The candidate must be autonomous and motivated.

To apply, send a CV, list of publications, cover letter, letters of recommendation and contacts of recent referees.
Contact: valerie.geoffroy@inserm.fr
Deputy Director Inserm UMR_S 1229-RMeS REGOS team
https://rmes.univ-nantes.fr/
Faculty of dental surgery, 1 place Alexis Ricordeau, BP 84215, 44042 Nantes Cedex 1

About FIDELIO

The EU-funded Innovative Training Network FIDELIO (https://www.fidelio-project.eu) aims to
train the next generation of scientists in order to tackle the challenges of diabetic bone
disease from various angles and with the newest technologies available. Interdisciplinary
training and implementation of innovative approaches are key. Within this consortium, we will
comprehensively unravel the genetic and environmental mechanisms that contribute to bone
fragility in diabetes, identify predictors and clinical markers for patient stratification, decipher
the underlying molecular mechanisms of bone fragility in diabetes, and establish potential
interventions through a personalised medicine approach.
The research programme will address different aspects of diabetic bone disease from the
viewpoints of epidemiology, genetics, miRNAs, microbiome, bone biology, bone
biomechanics and microstructure, preclinical and clinical research. It will utilise advanced
imaging and computational approaches, diabetes mouse models and access to clinical
cohorts and registry data to obtain a comprehensive overview of how these mechanisms
combine in diabetes to cause increased fracture risk.
With this interdisciplinary approach, we can explore the impact of biological pathways in
mouse models and/or humans, and interactions with diet, exercise and other exposures.
Collaborations with industry will allow early identification of IP, access to state of the art
technologies, and will complement the academic ESR training programme with
entrepreneurship and industrial mentoring.

About the host organization

The Universitaetsklinikum Hamburg-Eppendorf (UKE) is one of Europe s most modern
clinics. Here specialists from all fields of medicine and life sciences are brought together
under one roof. State-of-the-art medical technology, innovative information technology, and
architecture created with the provision of medical care at the forefront, all with the aim of
optimally supporting doctors, nursing staff, and therapists. With a strong focus on research,
ideal conditions for the interlinkage between modern medicine, translational research, and
teaching are found at the UKE. More than 11,000 employees work around the clock with the
singular aim of providing the best medical care to our patients. Webpage:
https://www.uke.de/english/index.html
The Heisenberg Research Group (Busse lab) at the Department of Osteology and
Biomechanics focusses on bone quality assessment. Basic, translational and clinical
research is performed on multiple aspects of musculoskeletal health. Webpage:
https://www.uke.de/english/departments-institutes/institutes/osteology-andbiomechanics/
index.html
The Busse lab has expertise in osteoporosis, musculoskeletal disorders, bone matrix
quality analysis, high-resolution imaging and biomechanical testing. The group consists
of an international, multidisciplinary team with backgrounds in the fields of biology, chemistry,
physics, medicine, bioengineering and computational analysis all working together to address
key questions in musculoskeletal health.

Task description

Your PhD project:
The candidate will focus on bone strength in diabetic bone with the help of computational
models. The project will involve the following tasks: i) investigate how microdamage, crosslinking
and bone porosity affect bone quality in diabetes. Therefore, the ESR will work on
biomechanical testing as well as micro-CT imaging of bone specimens. ii) The candidate will
work on the development of a micro-FE model incorporating matrix properties to predict
fracture risk in diabetic bone. Therefore, the candidate will also focus on data obtainment
from murine diabetes models (collaboration with the University of Dresden, Germany). iii)
The candidate will work on the development of a qCT-based FE model incorporating crucial
bone quality factors utilizing data from human diabetic cohorts and age-matched controls in
order to build and validate clinical FE models.
Secondments:
You will embark on secondments to other FIDELIO partners (SCANCO (CH), ETH (CH), IBM
(CH); UCBM (IT) or SDU (DK)) to access experimental models and tools or receive additional
training from experts in the field. This will include a focus on computational models and
methods, high-resolution image processing and micro-FE modelling. Further, code
optimisation and/or acceleration of computation as well as testing FE models using
prospective or retrospective fracture data. Total secondment time is 5 months.
Benefits of working in an ITN:
– You will be working within our international, interdisciplinary team of >30 researchers
with experience in a broad range of sciences.
– You will get in contact with the other members of this international consortium and will
benefit from the joint training platform to develop skills necessary for developing a
thorough understanding of the mechanisms of diabetes and the bone metabolism and
for obtaining industry skills.

Profile and requirements

• Applicants must hold an MSc or equivalent in the field of biomedical engineering,
  biomechanical engineering, bioinformatics, computer sciences or a related program
  Applicants must have knowledge and/or experience in finite element modelling
  Willingness to work with bone specimens from animals and humans is a prerequisite
• Applicants can be of any nationality
• Applicants must have an ability to understand and express themselves in both written and
  spoken English to a level that is sufficiently high for them to derive the full benefit from the
  network training
• Applicants must be eligible to enroll on a PhD program at the host institution (or a
  designated university in case the host institution is a non-academic organization)

In addition:
H2020 MSCA Mobility Rule: researchers must not have resided or carried out their main
activity (work, studies, etc.) in the country of the host organization (Germany) for more than
12 months in the 3 years immediately before the recruitment date. Compulsory national
service, short stays such as holidays, and time spent as part of a procedure for obtaining
refugee status are not taken into account.
Eligible researchers must not have spent more than 12 months in the 3 years immediately
prior to the date of selection in the same appointing international organisation.
H2020 MSCA eligibility criteria: Early Stage Researchers (ESRs) must, at the date of
recruitment by the host organization, be in the first four years (full-time equivalent research
experience) of their research careers and have not been awarded a doctoral degree. Full-
Time Equivalent Research Experience is measured from the date when the researcher
obtained the degree entitling him/her to embark on a doctorate (either in the country in which
the degree was obtained or in the country in which the researcher is recruited, even if a
doctorate was never started or envisaged).

Benefits

• You will be employed by the host organization for 36 months (with the option of an
  extension of the employment later on).
• A competitive salary plus allowances. Moreover, funding is available for technical and
  personal skills training and participation in international research events.
• You will benefit from the designed training program offered by the host organization and
  the consortium.
• You will participate in international conferences and secondments to other organizations
  within the FIDELIO network and in outreach activities targeted at a wide audience

Please find additional information in the Information package for Marie Curie fellows

Application

Interested candidates are invited to apply online at https://www.fidelio-project.eu/contact/.
Expected start date: August 2021
More information can be found on https://www.fidelio-project.eu

Additional information

We in the FIDELIO consortium value diversity and we commit to equal treatment of all
applicants irrespective of gender, sexuality, health status as well as social, cultural or
religious background.
For additional information about the research project and this individual position, please
contact:
Prof. Dr. rer. medic. Bj rn Busse
E-Mail: b.busse@uke.de

Department of Endocrinology, University Hospital of Odense, Odense, Denmark invites applications for a PhD position to study: The role of circulating and bone tissue microRNAs in the pathogenesis of type 2 diabetic bone fragility. The position is available for 3 years. The starting date is in summer 2021.

Job description
The project aim is to study the pathophysiology of bone fragility observed in patients with type 2 diabetes that leads to increased risk for fracture. Specifically, we will examine whether bone and circulating microRNAs (miRNAs) and miRNA signatures can serve as diagnostic and prognostic biomarkers for the severity of diabetic bone disease We will also identify miRNAs that play a role in the pathogenesis of diabetic bone disease.
The PhD fellowship is provided by the NovoNordisk foundation and it is a collaboration between the Steno Diabetes Centers of Odense and Alborg, iNANO center at the University of Aarhus and the Center for Musculoskeletal Biology and Medicine, University of California San Francisco (UCSF).
The primary workplace will be the Molecular Endocrinology and Stem Cell Research Unit at the Department of Endocrinology, Odense University Hospital which constitutes an international, ambitious, highly dynamic, and well-equipped working environment. The Unit has access to clinically relevant patient material and state-of-the-art facilities for stem cell phenotyping, flow cytometry, bioimaging, immunohistochemistry and transgenic mouse work. Part of the PhD will take place at UCSF, San Francisco, USA.

As a PhD student you will receive training both in molecular as well as in clinical/translational research: you will learn molecular biology technologies for isolation, characterization and studying the function of miRNA in vitro and in vivo. In addition, you will be trained in using in vivo assays of ectopic bone formation and will work with murine fracture models. You will also learn how to integrate biological data with clinical patient characteristics. During the US stay, you will learn different in vivo human bone imaging modalities such as high resolution peripheral quantitative computed tomography (HR-pQCT), and quantitative computed tomography (QCT). You will learn assessment of human volumetric bone density, bone microarchitecture, geometry and strength by using semi-automated contouring methods and by applying standard and extended cortical analysis techniques to existing HR-pQCT- and QCT-image datasets. In addition, you will receive training in clinical data collection and clinical data management, as well as clinical statistical training.

Qualifications

We are looking for highly motivated, dedicated and ambitious scientists with:
• An undergraduate Master degree in Life or Medical Science, Molecular Biology, Biochemistry, Biomedicine or Endocrinology
• Documented expertise in cell biology and basic molecular biology technologies is an advantage.
• Good communication and collaborative skills and an interest in working in an interdisciplinary environment
• Proficiency in spoken and written English

For further information about the position, please contact: Dr. Moustapha Kassem mkassem@health.sdu.dk and Dr. Ursula Heilmeier, Ursula.Heilmeier@ucsf.edu

Candidates who are about to obtain these qualifications are also encouraged to apply.
The successful applicant will be employed in accordance with the agreement between the Ministry of Finance and AC (the Danish Confederation of Professional Associations). Applications – including the required attachments –should be sent to Dr. Kassem or Dr. Heilmeier directly via email. Only applications written in English will be accepted for evaluation. The application must contain (as pdf.- or docx.-files): • a motivation letter • a detailed CV • degree certificates (inclusive diplomata) • a complete and numbered list of publications and conference abstracts (if available) • a research plan detailing the candidate’s ideas and plans for her/his future research work.
• 2 letters of reference Incomplete applications with regard to the above-mentioned requirements will not be assessed.

Application deadline is June 15th 2021.
The University wishes our staff to reflect the diversity of society and thus welcomes applications from all qualified candidates regardless of personal background.

A position as postdoc is vacant at the laboratory of associate professor Kent Søe, Clinical Cell Biology, Pathology Research Unit, Department of Clinical Research, University of Southern Denmark, Odense, Denmark.

The position is handled in collaboration between the Department of Clinical Research and the Department of Molecular Medicine. The appointment will take place at the Department of Molecular Medicine, University of Southern Denmark.

The position is available from the 1st of April 2021 or as soon as possible thereafter. Subject to the approval of the granting authorities, the position is scheduled to a duration of up to maximum four years.

Please click on the button below for more information about this postdoc opportunity.

Applications are now open